Scanning Electron Microscopy of Cerebellar Cortex by Orlando Castejón

By Orlando Castejón

In a transparent exposition this inspirational ebook exhibits the in situ three-d morphology of cerebellar neurons, intracortical circuits and synaptic connections that underpin the functioning of cerebellar neurons in networks. It brings jointly in a single quantity a brand new view of the third-dimensional cytoarchitectonic association of the cerebellar cortex.

The e-book indicates the cerebellar extrinsic and intrinsic intracortical circuits shaped by means of mossy and mountain climbing fibers as uncovered through the cryofracture tools. The excessive measure of lateral collateralization of those fibers is additionally displayed delivering new insights at the info processing within the cerebellar cortex. in addition to, box emission excessive solution electron microscopy exhibits its capability contribution to the research of synaptic morphology. The concluding bankruptcy offers with the contribution of scanning electron microscopy to cerebellar neurobiology.

This monograph is an authoritative survey and a needs to for a person who's drawn to the constitution of the significant apprehensive approach. it is going to additionally entice an interdisciplinary viewers who desires to research extra approximately electron microscopy and neurocytology.

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Scanning Electron Microscopy of Cerebellar Cortex

In a transparent exposition this inspirational publication indicates the in situ three-d morphology of cerebellar neurons, intracortical circuits and synaptic connections that underpin the functioning of cerebellar neurons in networks. It brings jointly in a single quantity a brand new view of the three-d cytoarchitectonic association of the cerebellar cortex.

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Additional resources for Scanning Electron Microscopy of Cerebellar Cortex

Sample text

According to Fox, the axonal collaterals of Lugaro cells contact the basket cell bodies in the lower molecular layer. Palay and Chan-Palay (1974) reported two types of horizontal fusiform cells possessing different axonal pattern and distribution. One type gives rise to an axon directed downward to the granular layer and reaching the white matter as described by Ramon y Cajal (1955). Sahin and Hockfield (1990) used double label immunocytochemistry to identify and classify the Lugaro cells in cat cerebellum.

Hojo (1994) prepared specimens of human cerebellar cortex, by means of t-buty I alcohol freeze-drying device to examine the Purkinje cell somatic surface. Castej6n and Castej6n (1997) described, in detail, the SEM three-dimensional features of Purkinje cells and their synaptic relationship in the molecular layer. THREE-DIMENSIONAL MORPHOLOGY AND OUTER SURFACE Exploration of the Purkinje cell layer of teleost fishes, at higher magnifications by means of conventional SEM, and using a 20 nm high resolution scanning probe, shows the outer surface of Purkinje cells (Figures 45 and 46).

The freeze-fracture method also has been applied to the study of rat pineal body (Krstic, 1974) and confirmed communication between the pineal canaliculi and the pericapillary spaces. 4, overnight, in order to provide complete intracellular and extracellular proteinaceous cross-linking. 4, is used to completely remove the primary fixative by rinsing the tissue several times with gentle agitation. 4, for 1 hr and then rinsing in cacodylate buffer several times. Delicate Specimen Preparation A graded series of ethanol (30, 50, 70, 80, 90, 2 X 100%) is used to substitute tissue fluids, prior to wrapping individual pieces in preformed absolute ethanol filled parafilm cryofracture packets.

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Scanning Electron Microscopy of Cerebellar Cortex by Orlando Castejón
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