Biomedical electron microscopy : illustrated methods and by Arvid B. Maunsbach, Björn A. Afzelius

By Arvid B. Maunsbach, Björn A. Afzelius

This accomplished reference illustrates optimum instruction tools in organic electron microscopy in comparison with universal methodological difficulties. not just will the fundamental methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be offered, however the authors additionally exercise to demonstrate extra really expert recommendations similar to unfavourable staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted photo analysis.

  • Authored by way of the main leaders within the organic electron microscopy field
  • Illustrates either optimum and suboptimal or artifactual ends up in a number of electron microscopy disciplines
  • Introduces scholars on the way to learn and interpret electron micrographs

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Extra resources for Biomedical electron microscopy : illustrated methods and interpretations

Example text

FIGURE 1,7C Respiratory epithelium from rabbit following glutaraldehyde and osmium tetroxide fixations, resin embedding, and section staining with uranyl acetate and lead citrate, x 80,000. 7D Cytoplasm of a lymphocyte from a HIV-positive patient. The cell was fixed in glutaraldehyde and osmium tetroxide, resin embedded, and section stained with uranyl acetate and lead citrate, x 40,000. The identification of a biological structure requires a correlation to biochemical, physiological, or other data.

1966). Absorption of 1125-labeled homologous albumin by rat kidney proximal tubule cells: A study of microperfused single proximal tubules by electron microscopic autoradiography and histochemisty. J. Ultrastr. Res. 15, 197-241. Maunsbach, A. B. (1976). Cellular mechanisms of tubular protein transport. ), Vol. II, pp. 145-167. University Park Press, Baltimore. Maunsbach, A. , Ning, G. , and Nielsen, S. (1997). Aquaporin-1 water channel expression in human kidney. J. Am. Soc. Nephrol. 8, 1-14. Maunsbach, A.

Following Epon embedding, thin sections were prepared for electron microscope autoradiography using Ilford L4 Nuclear Research Emulsion. x 12,000. 9B A n electron micrograph of the apical part of the cell in the renal proximal tubule of Necturus maculosus following microperfusion with a solution of ferritin. The tubule was fixed with glutaraldehyde after 45 min and processed for electron microscopy, x 25,000. These figures illustrate the dynamics of endocytosis, including time sequence, direction of transport, and identification of involved cell components.

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Biomedical electron microscopy : illustrated methods and by Arvid B. Maunsbach, Björn A. Afzelius
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