Basic Techniques for Transmission Electron Microscopy by M. A. Hayat

By M. A. Hayat

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Crystals of O s 0 4 or iodine can also be used in the BEEM chamber. 25-g vial of O s 0 4 crystals. Osmication seems to be completed in 8 hr. The tissue turns greyish with these vapors and turns black after introduction of a fluid of some sort. For certain studies, vapor fixation is carried out in a chamber that is maintained at - 9 0 ° C and contains an open ampoule of O s 0 4 crystals. After an overnight fixation, the specimens are gradually brought to 4°C in a refrigerator. It should be noted that O s 0 4 vapor improves the preservation of the surface association of bacteria and yeast with the skin.

Rabbit) is anesthetized by intravenous pentobar­ bitone sodium (30 mg/kg body weight). A nylon catheter with a three-way stopcock is inserted in the right femoral artery with the tip between the left renal and coelic arteries. The aorta is dissected free so as to control the position of the tip of the catheter and to prepare the clamping of the aorta above the origin of the coelic artery, just below the diaphragm. Modified Ringer's solution containing 0 . 1 % procaine is perfused at a perfu­ sion pressure of 110 mmHg, which is equal to the systolic blood pressure.

Grids are placed in covered 100-mm glass Petri dishes for a short period to allow dissipation of residual osmium vapor before their poststaining and storage. Crystals of O s 0 4 or iodine can also be used in the BEEM chamber. 25-g vial of O s 0 4 crystals. Osmication seems to be completed in 8 hr. The tissue turns greyish with these vapors and turns black after introduction of a fluid of some sort. For certain studies, vapor fixation is carried out in a chamber that is maintained at - 9 0 ° C and contains an open ampoule of O s 0 4 crystals.

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Basic Techniques for Transmission Electron Microscopy by M. A. Hayat
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