Applications of Synchrotron Radiation: Micro Beams in Cell by Ari Ide-Ektessabi

By Ari Ide-Ektessabi

This booklet demonstrates the functions of synchrotron radiation in yes points of mobile microbiology, in particular non-destructive elemental analyses, chemical-state analyses and imaging (distribution) of the weather inside a phone. the fundamentals for realizing and purposes of synchrotron radiation also are defined to make the contents more straightforward to be understood for a large workforce of researchers in clinical and organic sciences who may not be acquainted with the physics of synchrotron radiation. the 2 major options which are mentioned during this ebook are the x-ray fluorescence spectroscopy (XRF) and the x-ray superb constitution research (XAFS). program of those suggestions in investigations of a number of very important medical fields, corresponding to neurodegeneration and different illnesses concerning mobilephone malfunctioning, are established during this book.

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Additional resources for Applications of Synchrotron Radiation: Micro Beams in Cell Micro Biology and Medicine

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J. W. Mayer, E. Rimini, “Ion Beam Handbook for Material Analysis”, Academic Press, 1977. 10. R. Tesmer, M. C. J. W. Mayer, “Handbook of Modern Ion Beam Materials Analysis”, Materials Research Society, 1995. 11. A. J. Annegarn, Nucl. Instrum. Meth. Phys. Res. B, 1996, 109, 209. 12. Z. X. Y. Tao, Chemom. Intell. Lab. , 1996, 32, 83. 13. A. Aarnio, H. Lauranto, Nucl. Instrum. Meth. Phys. Res. A, 1989, 276, 608. 14. B. Vekemans, K. Janssens, L. Vincze, F. Adams, P. van Espen, Spectrochim. Acta B, 1995, 50, 149.

7) where W1 , . . , Wn are the weight fraction of components. In the experimental configuration shown in Fig. 8) where φ is the incidence angle and ρ is the density of the sample. 9) Fig. 7. 5 Quantitative XRF Analysis 21 where Pi is the probability that a characteristics X-ray line of element i is emitted, which is determined mainly by the ionization cross section and the fluorescence yield. 11) λf is the wavelength of the fluorescence X-ray, and ϕ is the take-off angle of the fluorescent X-ray detector.

A small dose of acetic acid was pipetted onto the dish. After 10 min, the methyl green solution was removed from the dish followed by Fig. 3. 2 Elemental Images of Single Macrophage Cells 51 washing in distilled water. The light microscopic photograph of macrophage cells stained by the methyl green solution is shown in Fig. 2, where it can be observed that the nucleoli in the nucleus of the macrophage were stained green. Similar procedure was followed for hematoxylin-eosin staining. After being rinsed and dried, the dish was wetted by phosphate buffered saline (PBS) solution and hematoxylin solution added into the dish.

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Applications of Synchrotron Radiation: Micro Beams in Cell by Ari Ide-Ektessabi
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